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文献和实验Expression Cloning of Human B Cell Immunoglobulins
characterization of the human antibody repertoire on a single cell level through the generation of recombinant monoclonal antibodies from single primary human B cells of defined origin. This protocol offers a detailed description of the method starting
Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus
Hepatitis B viruses (HBVs) are characterized by their high species and tissue specificity. Because of the large number of patients chronically infected with HBV, the development of new treatment strategies remains a major goal but is hindered
Human In-Cell scFv Library from Infiltrating B Cell
to the study of the antibody repertoire formed in vivo during, for example, autoimmune diseases. We describe here, the use of the in-cell PCR together with Cre-recombination applied to human B cells to obtain in situ pairing of the variable (V
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