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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
with non-immune reactivity for immunoglobulin G. J. Immunol. 111:1401-1406. Richman, D. D., Cleveland, P. H., Oxman, M. N., and Johnson, K. M. 1982. The binding of staphylococcl protein A by the sera of different animal species. J. Immunol. 128
them individually with different fluorochrome conjugated secondary antibodies? (FITC-conjugated anti-Mouse IgG 1 will label both!) The biotin-avidin system may provide an answer, if one antibody can be biotinylated: label with the non-biotinylated mouse antibody
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