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2 µg
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文献和实验Alanine Transaminase Activity Assay
within two months. In the assay (optional), add 5-10 µl positive control and adjust the final volume to 20 µl/well with Alanine Transaminase Assay Buffer. 2. Standard Curve Preparation: 1) Colorimetric assay: Dilute the Pyruvate
Assays of Pyruvate Dehydrogenase Complex and Pyruvate Carboxylase Activity
Pyruvate dehydrogenase complex (PDC) and pyruvate carboxylase (PC) are mitochondrial enzymes that provide the initial steps of the two main alternatives for pyruvate metabolism: oxidative decarboxylation vs. anaplerotic carboxylation
Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
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