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文献和实验Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families
, for the low Km component. By contrast, for the high Km component, the apparent Km is approx 150 �M and the maximal specific activity is 0.35–0.95 nmol/min/mg microsomal protein (2 ). Experiments with recombinant human P450s have shown that multiple enzymes
High-Level Baculoviral Expression of Lysosomal Acid Lipase
Lysosomal acid lipase (LAL, EC 3.1.1.13) is a hydrolase essential for the intracellular degradation of lipids derived from plasma lipoproteins (1 ). LAL is synthesized in most cells and tissues of the human body, including fibroblasts
(5 ). Immunoinhibition experiments with heterologous and antihuman CYP4Al l antibodies have further established that CYP4Al l contributes a major fraction of the lauric acid 12-hydroxylase activity in human liver microsomes (3 , 5 , 6 ). Accordingly, microsomal lauric
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