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文献和实验Somatic Cell Nuclear Transfer in the Mouse
cell type for NT, in which donor nuclei are directly injected into the oocyte using a piezo-actuated micromanipulator. In particular, we focus on a new, more efficient mouse cloning protocol using trichostatin A (TSA), a histone deacetylase (HDAC
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
) GST‐3C (prepared in‐house) or PreScission 3C protease (GE Healthcare Life Sciences, cat. no. 27‐0843‐01) Histone H1 (Sigma
TSA Treatment of Mammalian Cells
Introduction Prolonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor, trichostatin A, specifically affects pericentric heterochromatin. Relocation of these regions towards the nuclear
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