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文献和实验Microtubule Spindowns for Visual Analysis
X stock and stored at 4¡C) Fix : 1% glutaraldehyde in BRB80 (prepare from a 50% glutaraldehyde stock just prior to use) Cushion : BRB80 + 10% (v/v) glycerol Spindown tubes with 12 mm coverslips (Evans et al. J. Cell Biol., 1985) HB-4/HB
RNA Whole Mount In Situ Hybridization
temperature. Blocking step: For chick embryos, block with 10% heat-inactivated sheep serum in TBST for at least one hour at room temperature. For mouse embryos, block embryos by incubating at room temperature for 60-90 minutes in blocking
Microtubule Spindowns for Visual Analysis
Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) Fix : 1% glutaraldehyde in BRB80 (prepare from a 50% glutaraldehyde stock
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