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文献和实验Microtubule Spindowns for Visual Analysis
X stock and stored at 4¡C) Fix : 1% glutaraldehyde in BRB80 (prepare from a 50% glutaraldehyde stock just prior to use) Cushion : BRB80 + 10% (v/v) glycerol Spindown tubes with 12 mm coverslips (Evans et al. J. Cell Biol., 1985) HB-4/HB
Microtubule Spindowns for Visual Analysis
Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) Fix : 1% glutaraldehyde in BRB80 (prepare from a 50% glutaraldehyde stock
Harvesting lymphocytes from Peripheral Blood
from the whole blood ・ Add 250ml of DMEM with 10% Fetal Goat Serum(FGS) to 250ml of whole blood to block nonspecific binding ・ Incubate at 4°C for 10 minutes ・ Add 1mg of appropriate antibodies to the specimen (usually about a million
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