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文献和实验Mouse p27 PCR Using Gitschier Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGTGCCATCTCTAT) produce a 0.5kB PCR product. Wildtype allele: mgK-3 (above) plus mcK-5 (GAGCAGACGCCCAAGAAGC) produce ~1kB product. Stock Solutions
with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Improved HSV-1 Amplicon Packaging System Using ICP27-Deleted, Oversized HSV-1 BAC DNA
-1-based vectors. Two distinct types of vectors, recombinant and amplicon, have been reported. Recombinant HSV-1 vectors use most of the viral genome as the vector backbone and can carry one or more gene(s) of interests inserted in place of the viral genes
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