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文献和实验Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons
implicated in dopaminergic cell death and for the evaluation of potential dopaminergic neuroprotective agents, including neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat mesencephalic cell cultures
Experimental Systems to Study Neurotrophic Factor Effects on Rat Brain Cells
Nerve growth factor (NGF) was discovered and the concept of neurotrophic factors as retrograde messengers was developed in studies on the peripheral nervous system (PNS). Initial descriptions of biological effects of newly discovered grow
Production of Recombinant Neurotrophic Factors
factor (NGF), and its related protein family members, brainderived neurotrophic factor (BDNF or NT-2), and neurotrophin3 (NT.-3). The potential of producing mg and larger quantities of these agents will expedite our understanding of their functions
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