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文献和实验Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons
and their application to evaluating the neuroprotective action of brain-derived neurotrophic factor.
Neural Differentiation of Human Adipose Tissue-Derived Stem Cells
) chemical induction, and neurosphere formation in combination with the cytokines, such as brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF). For in vivo neurogenic induction, ASCs are treated with BDNF and bFGF to form
culture media containing recombinant human stem cell factor (rhSCF), rhIL-6, and rhIL-3 (first week only). Weekly hemidepletions and the removal of adherent cells and/or debris enables the investigator to obtain HMC cultures, identified by Wright-Giemsa
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