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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
botulinum neurotoxin serotype FI, 240 mg/kg cells307Clostridium botulinum neurotoxin serotype A binding domainI, 2.4 mg total[59]Clostridium tetani Tetanus toxin fragment CI, 12 g/L[60]Diphtheria anti-human anti-T cell immunotoxinS, 10 mg/L, α-MF
Studies of the Ubiquitin Proteasome System
) 10× in vitro translation (IVT) buffer (Promega) Rabbit reticulocyte lysate or wheat germ extract (Promega) Amino acid mixture (Promega) lacking methionine (−Met), cysteine (−Cys) or lysine (−Lys) depending on amino acid
Strategies to Optimize Protein Expression in E. coli
, D.R., Timotijevic, G.S., and Maksimovic, V.R. 2009. Successful production of recombinant buckwheat cysteine‐rich aspartic protease in Escherichia coli. J. Serb. Chem. Soc. 74:607‐618.
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