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文献和实验Prediction of Protein‐Protein Interaction Networks
Figure 8.2.3 One method of gene fusion. Individual proteins, A and B, from one genome can often be found as a single fused protein, C, in another genome. The finding of such a fused protein suggests
, where analysis of any peptide within the protein can be informative, phosphorylation site analysis requires that the phosphorylated peptide be analyzed. This means that considerably more protein is required for analysis. In addition, phosphorylation can alter
Strategies to Optimize Protein Expression in E. coli
, R.B., and Waugh, D.S. 2001. Single amino acid substitutions on the surface of Escherichia coli maltose‐binding protein can have a profound impact on the solubility of fusion proteins. Protein Sci. 10:622‐630.
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