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- 2026年04月15日
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文献和实验Basic procedures for bacteria culture
DNA specific endonuclease I. Mutation shown to improve yield and quality of DNA from plasmid minipreps. F' F' episome, male E. coli host. Necessary for M13 infection. galK Inability to utilize galactose. galT Inability to utilize galactose
methyladenine as well as 5mC (as with bacterial DNAs). (12) 5. XL1-Blue MRF'=D(mcrA)182, D(mcrCB-hsdSMR-mrr)172,endA1, supE44, thi-1, recA, gyrA96, relA1, lac, l-, [F' proAB, lacIqZDM15, Tn10(tetr)]. Host Mutation Descriptions:
DNAs). (12) 5. XL1-Blue MRF'=D(mcrA)182, D(mcrCB-hsdSMR-mrr)172,endA1, supE44, thi-1, recA, gyrA96, relA1, lac, l-, [F' proAB, lacIqZDM15, Tn10(tetr)]. Host Mutation Descriptions: ara Inability to utilize arabinose. deoR Regulatory gene that allows
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