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文献和实验Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
The Rat Aortic Ring Assay for In Vitro Study of Angiogenesis
of angiogenesis has been shown in pre-clinical studies to suppress tumor growth (2 –4 ). Currently, the use of anti-angiogenic therapy in cancer is under intensive investigation (5 ). Several in vitro and in vivo assays are available to study angiogenesis
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