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文献和实验Synchronization of Mammalian Cell Cultures by Serum Deprivation
from newborn rat brain by serum deprivation. The procedure is similar, and generally applicable, to other mammalian cells. This protocol consists essentially of two parts: (1) proliferation of astrocytes under optimal conditions in vitro until reaching desired
Preparation of Mixed Glial Cultures from Postnatal Rat Brain
separately in serum or serum-free media to yield large quantities of the desired glial cell subpopulations with a high degree of purity in the range of 96–99%. These cell culture models have been used extensively for performing biochemical
The Rat Aortic Ring Assay for In Vitro Study of Angiogenesis
, each with its own advantages and limitations (6 ). In vivo assays simulate the natural process but are often complicated by inflammatory host responses that may interfere with angiogenesis. Variables are more likely to be controlled using in vitro assays
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