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文献和实验Studies of the Ubiquitin Proteasome System
both in vitro and in cells. Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit. Keywords: Ubiquitin; ubiquitylation; proteasomal degradation; protein
Global Analysis of Ubiquitination
The covalent attachment of the small protein ubiquitin to other proteins is known to control a host of biological pathways and is emerging as an important regulatory factor in various processes specific to the nervous system. Ubiquitination
Strategies to Optimize Protein Expression in E. coli
Bao, W.J., Gao, Y.G., Chang, Y.G., Zhang, T.Y., Lin, X.J., Yan, X.Z., and Hu, H.Y. 2006. Highly efficient expression and purification system of small‐size protein domains in Escherichia coli for biochemical characterization. Protein Expr
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