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- Norgenbiotek
- NGB-27650
- 2025年07月14日
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文献和实验实验试剂 96–100% ethanol 2–mercaptoethanol 70% ethanol (in RNase-Free Water) PBS (for samples with >107 cells) 实验设备 1.5 mL RNase-free microcentrifuge tubes RNase-free pipet tips
the collection rack with clean 1.5 ml screw-capped tubes into the manifold. Clamp the manifold with the white clamps, and apply 300 ul of 10:1 TE buffer heated to 65degC and pull the eluted DNA through at 5 in. Hg. After the liquid has filtered through, raise
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
III and gently mix by inverting several times until a flocculent white precipitate forms. Centrifuge at $10,000 × g for 10 minutes at room temperature. 9. CAREFULLY aspirate and add the clear supernatant to a clean HiBind® DNA mini column
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