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文献和实验Since the inception of affinity chromatography 30 years ago (1 ), it has developed into a powerful tool mainly for the purification of proteins. It is based on the reversible formation of a tight binding complex between a ligand, immobilized
Affinity Purification of Monoclonal Antibodies
preparations are needed. Affinity chromatography, which relies on the specific interaction between an immobilized ligand and a particular molecule sought to be purified, is a well-known technique for the purification of proteins from solution. Three different
Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
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