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文献和实验Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine
L -Ornithine decarboxylase (EC 4.1.1.17) (ODC) catalyzes the conversion of L -ornithine to putrescine and CO2 . ODC is dependent on pyridoxal 5′-phosphate (PLP) and thiol-reducing agents for activity (1 ). At least two key active site
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing
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