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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Metastatic Orthotopic Mouse Models of Lung Cancer
. McLemore et al. (3) subsequently developed a second model by injecting lung tumor cells via an intrathoracic route into the pleural space. This model seems similar to the intrabronchial model in that extensive local growth occurs with little metastatic
Freeze storage of human embryonic stem (hES) cells has not proven effective using the methods employed for mouse ES (mES) cells, while rhesus ES (rhES) cells are only modestly effectively frozen using common mES cell methods. Because human
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