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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
. Griffin, B.A., Adams, S.R., and Tsien, R.Y. 1998. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281:269–272.
resonance energy transfer (BRET) allows monitoring of protein?protein interactions in real time in living cells. One candidate interacting protein is fused to a luminescent energy donor, such as Renilla luciferase, and the other to a fluorescent energy
. These vectors permit the insertion of single or multiple genes for expression, or the production of proteins with specific peptide tags that aid subsequent protein purification. A table listing the transfer vectors also included information on the parental virus
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