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文献和实验E.Z.N.A.TM Plant DNA Maxi Protocol For Dry Specimens
x g 2. Centrifuge with swinging-bucket rotor at room temperature capable of 4000xg. 3. Nuclease-free 50 ml high speed centrifuge tubes (such as Nelgen polycarbonate tube Cat#3118-0050) and 50ml centrifuge tubes capable of 4000 x g
Yeast IF with Methanol/Acetone Dehydration
for 10 min. I suggest you do each sample in duplicate. Aspirate off most of the liquid but not all (do not dry the slides) and plunge, this is important, plunge the slide into a coplin jar that is surrounded by dry ice for 6 min. then plunge
Yeast IF with Methanol/Acetone Dehydration
off most of the liquid but not all (do not dry the slides) and plunge, this is important, plunge the slide into a coplin jar that is surrounded by dry ice for 6 min. then plunge into another coplin jar that contains acetone and is also surrounded by dry ice for 30sec
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