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文献和实验Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
, including rat hybridomas (6 –8 ). However, some of the restriction sites (in particular Pst I and Barn HI) introduced for subsequent cloning were found to be frequently present as internal sites in the DNA coding for mouse-antibody-variable domains
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
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