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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
Immunohistochemistry: Fluorescence Protocol 2.0
temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 , IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5% human AB serum. Cells are ready for analysis by flow cytometry
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