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999
- 规格：
10 mg
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
RNA Purification Protocol for 10-20mg of Animal Tissue
. Fresh tissue can also be used. Work quickly and keep the sample on ice at all time. 2. Add 10-20 mg frozen ground tissue or fresh tissue to a 1.5 mL microtube containing 600ul RCL Buffer. Homogenize throughly using a microfuge tube pestle (Product
do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
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