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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Cell Surface Immunofluorescence Staining Protocol
pellet in residual buffer and add previously determined optimum concentrations of anti-species immunoglobulin fluorochrome conjugated secondary antibody (e.g. FITC anti-mouse Ig) and incubate in the dark for 15-20 minutes. If using a biotinylated primary
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