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文献和实验Th17 Polarization of Mouse CD4 Cells
of anti-mouse CD28, clone 37.51(5μg/mL),IL-6 (50ng/mL),TGF-β1 (1ng/mL), anti-mouse IL-4 (10μg/mL), anti-mouse IFN-γ (10μg/mL), and 300 nM of FICZ. Alternatively, mouse IL-23 (5ng/ ml) can be used in place of FICZ. 3) On day
may require different culture methods. For example, mouse embryonic fibroblasts (MEFs) may be used as an alternative to gelatin, in combination with LIF (Nagy et al. 2003 ). 2. Wash the cells twice with 1X HBSS. 3. Add 2 mL
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
is a unique structure in vertebrates. Neural crest cells play important roles in the formation of organs that characterize the vertebrate body plan. In this unit, we describe a primary culture method for mouse mesencephalic neural crest cells. The neural crest
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