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文献和实验Introduction . We hope that this information will be useful to investigators who are unfamiliar with mouse breeding or who are breeding transgenic or gene targeted mice for the first time. These suggestions are based on our experience
Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine
dry 4) Block slides in PBST + 1% BSA for 20 min at RT 5) Incubate slides in monoclonal anti-5-mC (1:1000 dilution in PBS) for 1h at 37oC in a humid chamber 6) Wash in PBST 2x5min 7) Incubate slides in Biotin anti-mouse IgG (1:500 dilution
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