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文献和实验Preparation and use of Zamboni’s-fixed Frozen Sections in Immunohistochemistry
33.77g Na2H PO4. 7H2O. 1000ml distilled H2O). This fixative is stable at 25oC for 12 months. PROCEDURES 1. Cut 7μm thick sections of frozen tissue and fix immediately in Zamboni’s fixative for 10 minutes. 2. Wash 3 x 10 minutes in Tris
T-cell dependent antigen specific in situ staining (Pe)
, Sigma A-6926, dissolved in 2.5 ml NN-DM formamide) 50 ml acetate buffer pH 5.5 (52.5 ml of 0.1M acetic acid solution + 196.5 ml of a 0.1M Na acetate solution, bring to 500 ml) 25 µl H 2 O 2 30%. Shake well Filter with a 45µm filter
was performed in a glass 100-ml beaker as follows. At intensive stirring, 20 drops of NaOH solution (5 M) were added to AgNO3 solution (500 mg) in Milli-Q water (60 ml), and dark brown precipitate formed immediately. Approximately 2 ml of 30% NH4 OH solution
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