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文献和实验Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 9. Secondary antibody to use with goat polyclonal reagent: donkeyanti-goat IgG-horseradish peroxidase (HRP) (sc-2056;Santa Cruz Biotechnology). 10. Secondary antibody to use with rabbit polyclonal reagent: donkey anti-rabbit IgG-HRP (#NA934V, GE
Dual- and Triple-Color Fluorospot
is the availability of additional amplification systems compatible with, e.g., FITC/anti-FITC and biotin/avidin and, in particular, automated readers designed for the analysis of three-colored spots. One amplification strategy that has been evaluated is to use
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