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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Abstract Table of Contents Materials Figures Literature Cited Abstract The mouse
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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