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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
reactions. Figure 1 . a) Genomic DNA extracted from whole blood of the sheep using the rapid isolation method on agarose gel; Lanes 1 to 5 and 6 to 10: DNA extracted from whole blood stored at -20ºC and +4ºC for one year, respectively. b
Purification Mix. 2. Preparing the Lysate Follow the procedure below to prepare a lysate from the 50-100 µl blood sample. 1) Transfer the 50-100 µl blood sample to a sterile microcentrifuge tube or a 96 x 2 ml deep well plate
Extraction of genomic DNA from whole blood
should be sterile. Buffer A should be autoclaved prior to addition of Triton-X-100. Sterile filtering of solutions instead of autoclaving is a better option. Procedure 1.Add 1 volume of buffer A to 1 volume of blood and 2 volumes of cold, sterile, distilled
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