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文献和实验Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Fusion of Mouse, Rat, or Hamster Cell
the pellet in HAT medium to 5 x 105 cells/ml. Plate 200 ml/well to 96 well plates. Incubate at 37o C, 8-10 CO2 for 5-7 days.
staining (normal): platelets and megakaryocytes CD42d Aka platelet GP V CD42d mouse monoclonal antibodies are used to detect auto- and allo-anti-platelet antibodies Co-expression studies suggest that CD42d may be essential
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