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文献和实验Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
the enzyme β-galactosidase, which cleaves the substrate X-gal (5-bromo-4-chloro-3-indolyl-β-d -galactoside) to produce a blue color reaction. The green fluorescent protein (GFP) from the jellyfish Aequorea victoria gives a natural green fluorescence
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