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文献和实验secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
RNase) Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS with 0.1% sodium azide. Label with the FITC-conjugated antibody of interest in cold PBS with 2% FBS and 0.1% sodium azide. Wash cells
Flow Cytometry Assay to Measure Internalization of GPCRs
, you must rinse the pellet with media to get rid of any residual chelating agents.8) Label receptors with fluorescent antibody -resuspend pellet in 500 µL of media containing 10 mg/mL FITC-M1 (M1 which has been covalently bound to the FITC fluorophore (Molecular
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