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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
297-473-0
- 规格:
L4130-100MG

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文献和实验Methylation of Fatty Acids (Kropinski Method)脂肪酸甲基化
nmoles/100ml (usually 10mg of C15 in 100ml of reagent.Weigh 10mg of whole cells or 1 mg of LPS into a clean screw-cap tube.Add 1ml of MeOH-HCl reagent/internal standard into each tube and vortex.Heat at 100oC for 20 minutes. (Note: each tube
Methylation of Fatty Acids (Kropinski Method)
of 400 nmoles/100ml (usually 10mg of C15 in 100ml of reagent. Weigh 10mg of whole cells or 1 mg of LPS into a clean screw-cap tube. Add 1ml of MeOH-HCl reagent/internal standard into each tube and vortex. Heat at 100o C for 20 minutes
, and the results observed after 3 to 5 minutes. PASSIVE AGGLUTINATION: Passive agglutination is performed using tanned sheep red blood cells and P. aeruginosa LPS (heated for 1 hour at 100o C prior to use). Sheep erythrocytes are washed three times
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