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DNA pol θ rabbit pAb

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  • ¥900
  • AAB
  • 上海
  • A13661
  • 2025年07月10日
  • IHC-p,ELISA
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      The antiserum was produced against synthesized peptide derived from human POLQ. AA range:181-230

    • 形态

      Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    • 保存条件

      -20°C/1 year

    • 克隆性

      Polyclonal

    • 适应物种

      Human

    • 保质期

      1年

    • 宿主

      Rabbit

    • 应用范围

      IHC-p,ELISA

    • 浓度

      1 mg/ml

    • 靶点

      POLQ

    • 抗体英文名

      DNA pol θ rabbit pAb

    • 抗体名

      DNA polymerase theta

    • 规格

      50ul

    POLQ (Polymerase (DNA) Theta) is a Protein Coding gene. Among its related pathways are Platinum Pathway, Pharmacokinetics/Pharmacodynamics and DNA Double-Strand Break Repair. GO annotations related to this gene include nucleic acid binding and damaged DNA binding. An important paralog of this gene is SNRNP200. NA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed: 25642963, PubMed: 25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed: 25642963, PubMed: 25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed: 25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed: 25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed: 18503084, PubMed: 21050863, PubMed: 22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed: 14576298, PubMed: 18503084, PubMed: 19188258, PubMed: 24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).

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    图标文献和实验
    相关实验
    • DNA末端修饰原理

      聚合酶活性和3` → 5`外切酶活性DNA pol Ⅱ:5` → 3`聚合酶活性及3` → 5`外切核酸酶活性.DNA polⅢ: 由10 个亚基组成,分别为α,ε,θ,τ,δ,δ`,β,κ及ψ.是原核生物体内真正起复制作用的酶.α亚基: 5` → 3`聚合酶活性ε亚基:3` → 5`外切酶,校对和编辑θ为装配必须大肠杆菌DNA聚合酶Ⅲ真核生物DNA聚合酶α,β,γ及δ四种,都有5`→ 3`聚合功能.α及δ参与核DNA复制;α:链合成的引发,δ:链的延长polδ;切除引物后填补空隙β:外切核酸酶γ:线粒体DNA

    •  DNA的生物合成

      点有结构上的特殊性,例如:大肠杆菌染色体DNA复制起始点Oric由422个核苷酸组成,是一系列对称排列的反向重复序列,即回文结构(palindrome),其中有9个核苷酸或13个核苷酸组成的保守序列,这些部位是大肠杆菌中DnaA蛋白识别的位置,大肠杆菌染色体DNA是环状双链DNA,它的复制是典型的“θ”型复制(由于形状像希腊字母θ)。从一个起点开始,同时向两个方向进行复制,当两个复制方向相遇时,复制就停止。而有些生物的DNA复制起始区是一段富含A・T的区段。这些特殊的结构对于在DNA复制起始过程中参与

    • ChIP 实验面面观,博士师兄吐血总结推荐

      法所替代。利用微球菌核酸酶的切割特性(在染色质核小体之间的 DNA 更容易被切开),可以快速温和地将染色质 DNA 切断,且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态,使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒,含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体(Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620)、阴性对照抗体、DNA

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