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- Norgenbiotek
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- 2025年07月09日
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文献和实验Maxiprep preparation of Plasmid DNA
实验概要 The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids.
minutes. 9. Pour off the supernatant containing plasmid DNA into a clean SS-34 tube. Add an equal volume of isopropanol (10 ml) and precipitate the nucleic acids on ice for 10 minutes. Centrifuge at 10 k × g for 10 minutes. 10. Aspirate
Maxiprep of plasmid DNA from E. coli
. BioCoder version Following is the Maxiprep of plasmid DNA from E.coli protocol in BioCoder, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol
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