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文献和实验Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum
for 5 min at room temperature. 11. To elute nucleic acid from the column, centrifuge at 8,000 x g for 1 min. Retain flow-through containing the nucleic acid. Place column into a second 1.5 ml tube.
Identification of Immunoreactive Viral Proteins
Several diagnostic tools are available for the identification of acute and latent viral infections. Although newly developed nucleic acid amplification methods, such as the polymerase chain reaction (PCR), have proved to be very useful
DNA Purification from Blood or Body Fluids
, plasma, serum, buffy coat, lymphocytes, and body fluids using a microcentrifuge. For total DNA purification using a vacuum manifold, see “Protocol: DNA Purification from Blood or Body Fluids (Vacuum Protocol)” on page 30. Important points
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