TGFB3基因敲除质粒

产品简介：

pLenti-TGFB3-sgRNA (TGFB3基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的TGFB3基因敲除的质粒(L00466 pLenti-TGFB3-sgRNA)、慢病毒(L00467 TGFB3 Knockout Lentivirus)、HEK293T细胞(L00468 TGFB3 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L00469 TGFB3 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L00470 TGFB3 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

TGFB3基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	TGFB3	7043	BC018503	NM_003239

 

About the gene
Official Symbol	TGFB3
Previous Symbol	ARVD1; ARVD
Official Full Name	transforming growth factor beta 3
Synonyms	ARVD; LDS5; RNHF; ARVD1; TGF-beta3
Location	14q24
Gene Type	protein_coding
Uniprot ID	P10600
Pathway/Library	cAMP & Ca Signaling Pathway Related Genes Library; Cell Proliferation Related Genes Library; Cell Cycle Pathway Genes Library
Gene Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Mutations in this gene are a cause of aortic aneurysms and dissections, as well as familial arrhythmogenic right ventricular dysplasia 1.