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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
165252-70-0
- 规格:
32499-50MG

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文献和实验Acid-guanidinium thiocyanate-phenol-chloroform RNA purification
with ribonucleases. This method describes preparation from small quantities (~ 50mg) of tissue. Appropriate scaling of the volumes involved can be performed to accomodate larger quantities. You will need: Guanidine isothiocyanate (Sigma) 1M sodium
Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions 上一篇:PREPARATION
ALPHA FACTOR ARREST AND RELEASE
) in media containing 0.1 mg/ml Pronase E (Sigma P-6911). I generally make a fresh10 mg/ml stock in media (i.e. YPD or YEP-raffinose) and then sterilize using a syringe filter unit. Filter sterilization is not necessary unless you anticipate prolonged
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