Sartorius赛多利斯14539--50------DC

BAC/PAC 克隆完全攻略,强烈申请加分!!

or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. Solutions P1 (filter sterilized, 4oC) 50mM Tris, pH 8 10 mM EDTA 100 ug/ml RNase A P2 (filter sterilized, room temp

In Situ Hybridization Protocols

Make a 10x stock of the salts and add fresh formaldehyde each time.   Alkaline Phosphatase Buffer: 100mM Tris, pH 9.5 50mM MgCl2 100mM NaCl 0.1% TWEEN 20

Anderson Lab In Situ Hybridization Protocols

up the following reaction in 50ul total volume: 5x Stratagene synthesis buffer 10ul 1M DTT (RNAase free) 0.5ul 10mM NTPs/digoxygenin-UTP 2.5ul RNAsin 10