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文献和实验Southern Hybridization Experiment Kit
This kit contains materials for six groups to perform Southern transfer and hybridization analysis using the included lambda DNA samples and biotinylated probe. The intellectual objective of the experiment is to determine the region
CellTrace CFSE Cell Proliferation Kit
in phosphate-buffered saline (PBS) or other suitable buffer to the desired working concentration (0.5–25 μM). Resuspend the cells gently in prewarmed (37°C) PBS containing the probe (prepared in previous step). Incubate the cells for 15 min
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
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