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- 上海莼试
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
58
- 英文名：
Heparin
- CAS号：
9005-49-6
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
25mg
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
产品描述：
分子式:C26H42N2O37S5
分子量:1134.93
溶解度:≥ 12.75mg/ml in H2O
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Heparin, a highly sulfated glycosaminoglycan, has been widely used as an injectable anticoagulant. Heparin is present only in a few tissues and species of the animal kingdom and in the form of granules inside organelles in the cytoplasm of special cells. Heparin might be involved in defense mechanisms against bacteria and other foreign materials [1].In vitro: Heparin (25-500 Nμ/ml) dose-dependently inhibited cellular aggregation and degranulation induced either by FMLP or by zymosan-activated serum. Heparin at the concentration of25 μg/ml specifically inhibited FMLP-dependent superoxide anion generation and chemiluminescence. Heparin inhibited all the aspects of the functional and metabolic granulocyte activation [2].In vivo: In the cells from heparin-treated rats or in the heparin-treated cells, specific binding of [125I]iodo-angiotensin II was decreased due to a decrease in both the number and the affinity of angiotensin II receptors. Heparin also decreased the maximum angiotensin Il-induced productionof aldosterone in the cells from heparin-treated rats and in the heparin-treated cells. Heparin interacted with adrenal angiotensin II receptors to inhibit the angiotensin Il-induced aldosterone production [3].References:[1]. Nader H B, Chavante S F, Dos-Santos E A, et al. Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates[J]. Brazilian Journal of Medical and Biological Research, 1999, 32(5): 529-538.[2]. Pasini F L, Pasqui A L, Ceccatelli L, et al. Heparin inhibition of polymorphonuclear leukocyte activation in vitro. A possible pharmacological approach to granulocyte-mediated vascular damage[J]. Thrombosis research, 1984, 35(5): 527-537.[3]. Azukizawa S, Iwasaki I, Kigoshi T, et al. Effects of heparin treatments in vivo and in vitro on adrenal angiotensin II receptors and angiotensin II-induced aldosterone production in rats[J]. Actaendocrinologica, 1988, 119(3): 367-372.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y64171 | 规格 | 25mg |
| CAS号 | 9005-49-6 | 分子量 | 1134.93 |
| 含量 | >98.00% | 别名 | N/A |
| 分子式 | C26H42N2O37S5 | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C26H42N2O37S5
分子量:1134.93
溶解度:≥ 12.75mg/ml in H2O
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Heparin, a highly sulfated glycosaminoglycan, has been widely used as an injectable anticoagulant. Heparin is present only in a few tissues and species of the animal kingdom and in the form of granules inside organelles in the cytoplasm of special cells. Heparin might be involved in defense mechanisms against bacteria and other foreign materials [1].In vitro: Heparin (25-500 Nμ/ml) dose-dependently inhibited cellular aggregation and degranulation induced either by FMLP or by zymosan-activated serum. Heparin at the concentration of25 μg/ml specifically inhibited FMLP-dependent superoxide anion generation and chemiluminescence. Heparin inhibited all the aspects of the functional and metabolic granulocyte activation [2].In vivo: In the cells from heparin-treated rats or in the heparin-treated cells, specific binding of [125I]iodo-angiotensin II was decreased due to a decrease in both the number and the affinity of angiotensin II receptors. Heparin also decreased the maximum angiotensin Il-induced productionof aldosterone in the cells from heparin-treated rats and in the heparin-treated cells. Heparin interacted with adrenal angiotensin II receptors to inhibit the angiotensin Il-induced aldosterone production [3].References:[1]. Nader H B, Chavante S F, Dos-Santos E A, et al. Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates[J]. Brazilian Journal of Medical and Biological Research, 1999, 32(5): 529-538.[2]. Pasini F L, Pasqui A L, Ceccatelli L, et al. Heparin inhibition of polymorphonuclear leukocyte activation in vitro. A possible pharmacological approach to granulocyte-mediated vascular damage[J]. Thrombosis research, 1984, 35(5): 527-537.[3]. Azukizawa S, Iwasaki I, Kigoshi T, et al. Effects of heparin treatments in vivo and in vitro on adrenal angiotensin II receptors and angiotensin II-induced aldosterone production in rats[J]. Actaendocrinologica, 1988, 119(3): 367-372.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
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文献和实验相关实验
动物的肥大细胞合成的粘多糖的一种。原来是从肝中得到的抗凝血的物质。作为医药是由肠粘膜等加工而得到的不含蛋白的制剂，已知在生物体内是和其他粘多糖一样，是以与蛋白结合的蛋白聚糖形态而存在，但不象硫酸软骨素那样的细胞外间质成分，似乎可以说它是细胞内成分。肝素的抑制作用必须有辅助因子（co－factor），但它是靠与蛋白质或其他带正电荷的高分子电解质的结合性而实现的，例如凝血酶和肝素辅因子结合成复合体则起抑制凝固作用。多糖链是由L-艾杜糖醛酸、D-葡萄糖醛酸、D-葡萄糖胺组成，硫酸
Heparin-Induced Thrombocytopenia
Heparin-induced thrombocytopenia (HIT) is caused by platelet-activating immunoglobulin (Ig) G antibodies that recognize multimolecular complexes of platelet factor 4 (PF4) bound to heparin or other polyanions. Most laboratory assays for HIT
2 variants are further subtyped into three categories depending on whether the mutation has its effect on the reactive site (RS), the heparin binding site (HBS) or has multiple or pleiotropic effects (PE). Thus, the last update of the antithrombin database
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Heparin
￥600 - 3200
