赛默飞世尔Thermo Fisher LB MEDIUM(L

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

separately. Isopropyl-β-D-thiogalactopyranoside (IPTG; 200 mg/mL) LB agar LB-ampicillin agar plates Alternatively, LB-kanamycin agar plates can be used (see Step 48). LB (Luria-Bertani) liquid medium Add the appropriate

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

Procedures for In Vitro Production of Bovine Embryos

Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM