赛默飞世尔Thermo Fisher LB MEDIUM (

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

separately. Isopropyl-β-D-thiogalactopyranoside (IPTG; 200 mg/mL) LB agar LB-ampicillin agar plates Alternatively, LB-kanamycin agar plates can be used (see Step 48). LB (Luria-Bertani) liquid medium Add the appropriate

A quick RNA mini-prep for Neurospora mycelial cultures

thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed

Preparing Antibiotics Stock Solution and Ampicillin Agar Plates

for stocks. Add 200 µl to 100 ml LB-Agar/LB-Medium (100 µg/ml). 200 µg/ml for Ampicillin works also fine. Preparation of Agar Plates  Agar Recipes: 10 g of NaCl 10 g of Tryptone 5 g of Yeast Extract 20 g of Agar