赛默飞世尔Thermo Fisher X25 RED STO

DNeasy 96植物技术：使用搅拌磨MM 300从新鲜植物叶片中分离DNA

DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting （使用前的重要注释） 1、Take time to familiarize yourself with the Mixer Mill MM 300 and the Centrifuge 4-15C/ Centrifuge 4K15C

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

from Genebridges. Electroporation was performed at 1.35 kV, 25 μF, 200 Ω using an Eppendorf electroporator (2510). Bacteria were incubated in 1 ml LB media at 37°C, 1100 rpm for 1�2 h before plating on agar plates conditioned with the appropriate antibiotic(s

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make