赛默飞世尔Thermo Fisher X25 RED STO

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell. Solutions are pipeted on one side and sucked out the other side by capillary action using Whatman #1 filter paper or a Kimwipe. Washes in flow

DNeasy 96植物技术：使用搅拌磨MM 300从新鲜植物叶片中分离DNA

DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting （使用前的重要注释） 1、Take time to familiarize yourself with the Mixer Mill MM 300 and the Centrifuge 4-15C/ Centrifuge 4K15C

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

from Genebridges. Electroporation was performed at 1.35 kV, 25 μF, 200 Ω using an Eppendorf electroporator (2510). Bacteria were incubated in 1 ml LB media at 37°C, 1100 rpm for 1�2 h before plating on agar plates conditioned with the appropriate antibiotic(s