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文献和实验Large-scale yeast transformation
must lack a crossbrace. We have a Sorvall T6000D, with H-1000B rotor. With this we use braceless microplate carriers (#11093) and also two earlier carriers that I removed the crossbraces from (#11065). The only apparent problem with removing the cross braces
Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
the air to escape. Microplate carriers for centrifuge that allow you to spin deepwell boxes: For this, the microplate carriers must lack a crossbrace. We have a Sorvall T6000D, with H-1000B rotor. With this we use braceless microplate carriers (#11093
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