赛默飞世尔Thermo Fisher LAB ARMOR B

【交流】染色体免疫沉淀技术详细步骤-young lab ！！！

EDTA  0.2 ml 0.5 mM  0.5M EGTA  0.1 ml 0.1%  10% Na-Deoxycholate  1.0 ml 0.5%  20% N-lauroylsarcosine  2.5 ml   ddH2O  93.2 ml       IV. Chromatin immunoprecipitation 1.  Add 100 µl antibody/magnetic bead mix from Part II, Step 7 to cell

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

Bisulfite sequencing of small DNA/cell samples

of Proteinase K (20 mg/ml) and 5 µg of yeast t-RNA (use 1.5 ml Eppendorf tubes ) Incubate 1h at 37°C Add 50 µl of phenol and 50 µl of chloroform/isoamylalcohol (24:1) Mix the samples gently by inverting the tubes Centrifuge